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About Me!

My name is Ghulam Mehdi Dar

D.O.B: July 4, 1996

Nationality: Indian

Languages: English, Hindi, Urdu, Kashmiri

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Work Experience

STUDY: Establishment of Central Molecular Lab in GIPMER to study diagnosis for precision medicine in order to understand the disease process and utilize it as a clinical research facility

 

TECHNIQUES LEARNED

  • PCR; RFLP-PCR, MS-PCR, RT-qPCR,

  • Electrophoresis; SDS-PAGE, AGE,

  • Statistical; Microsoft PowerBI, MedCalc, SPSS,

  • Protein-based: Immunohistochemistry,

  • Basic microscopy, Fluorescent microscopy

  • Literature review and drafting.

FEB 2023- PRESENT

SENIOR RESEARCH FELLOW

CENTRAL MOLECULAR LAB, GIPMER, NEW DELHI

DR. SUNDEEP SINGH SALUJA, PROF. DEPT. OF GI SURGERY, GIPMER

STUDY: Bioinformatics analysis of methylome status of circulating DNA in Acute Myocardial Infraction and exploration of its biomarker utility

 

TECHNIQUES LEARNED

  • Clinical; Phlebotomy, Patient history,

  • Nucleic acid-based: cfDNA Isolation, DNA (blood/tissue) and RNA isolation,

  • Quality check: Nanodrop, Qubit, Bioanalyzer.

  • PCR-based; MS-PCR, RFLP-PCR, Gradient PCR,

  • Literature review and drafting

JAN 2021-JAN 2023

RESEARCH ASSISTANT

CENTRAL MOLECULAR LAB, GIPMER, NEW DELHI

DR. BHAWNA MAHAJAN, PROF. DEPT. OF BIOCHEMISTRY

STUDY: Effect of Doxorubicin and Etoposide on COX4 expression in Breast Cancer Cell-line (MDA-MB 231) under Hypoxia

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TECHNIQUES LEARNED

  • Molecular Techniques: Handling of Breast Cancer Cell-line (MDA-MB-231), Western Blotting, DNA/RNA Isolation, cDNA Synthesis, Hypoxia-Induction, Agarose Gel Electrophoresis, SDS-PAGE, RT-qPCR, Nanodrop QC  

  • Computational Skills: Microsoft Word, Excel and Powerpoint, Research Methdology, Primer designing, EndNote Ref. Manager

JUL 2019 - MAY 2020

MASTERS PROJECT 

DEPT. OF HGMM, CENTRAL UNIVERSITY OF PUNJAB

DR. SANDEEP SINGH, ASSOCIATE PROFESSOR, DEPT. OF HGMM

STUDY: Basics of Cancer cell culture handling and Protein analysis

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TECHNIQUES LEARNED

  • Molecular Techniques: Handling of Breast Cancer Cell-line (MDA-MB-231), Western Blotting, Agarose Gel Electrophoresis, SDS-PAGE, MTT-Assay, Bradford Assay, Radiography  

  • Computational Skills: Microsoft Word, Excel and Powerpoint, Research Methdology

JUNE 2019 – JULY 2019

RESEARCH TRAINEE

DIVISION OF CANCER PHARMACOLOGY, CSIR-IIIM, SRINAGAR

DR. FAYAZ AHMAD MALIK, PRINCIPLE SCIENTIST, HOD

STUDY: Phytochemical analysis and medicinal properties of Cymbopogon citratus (Lemon Grass)

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TECHNIQUES LEARNED

  • Molecular Techniques: Plant Cell Culture, Bacteria Cell Culture, Agarose Gel Electrophoresis, Soxhlet Apparatus, Rotavapor, Phytochemical Extraction, Gram Staining, DNA isolation from Blood and Bacteria

  • Computational Skills: Microsoft Word, Excel and Powerpoint, Primer Designing, Research Methdology

JULY 2017 - MAY 2018

BACHELORS PROJECT

CHANDIGARH UNIVERSITY, MOHALI, DEPARTMENT OF BIOTECHNOLOGY

DR. MAHAVIR JOSHI, ASSOCIATE PROFESSOR, CU

STUDY: Basics of Cell culturing and Agrobacterium Mediated Transformation of Fungus (C. lindemuthianum)

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TECHNIQUES LEARNED

  • Molecular Techniques: DNA Isolation from Plants, Agarose Gel Electrophoresis, bacterial Cell Culture, Agrobacterium-mediated Gene Transformation

  • Computational Skills: Microsoft Word, Excel and Powerpoint, Protocol management, Research Methdology

MAY 2017 - JULY 2017

RESEARCH TRAINING

PLANT PATHOLOGY LAB,SKUAST-K, SRINAGAR

PROF. ALI ANWAR, HOD DIVISION OF PLANT PATHOLOGY

Education

PhD IN BIOTECHNOLOGY

APRIL 2024 - PRESENT

JAMIA MILLIA ISLAMIA, NEW DELHI

CGPA: PURSUING

M.Sc IN LIFE SCIENCES: MOLECULAR MEDICINE

JULY 2018 - MAY 2020

CENTRAL UNIVERSITY OF PUNJAB, BATHINDA

CGPA: 7.11 (GRADE A)

B.Sc IN BIOTECHNOLOGY

AUG 2015 - MAY 2018

CHANDIGARH UNIVERSITY, MOHALI

CGPA: 8.59 (GRADE A+)

Participations and Awards 

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Memberships

  • April 2024-Present: Canadian Cardiology Society member (ID:157182)

  • September 2023-Present: Baishideng Reference Citation Analysis (RCA) member (ID: 00039045

  • February 2022-Present: Global Outreach - Student Member of the American Society for Microbiology Membership ID: 200338928 

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